There were 12 replicate wells per dilution in both 96-well and 384- well plates. 303 unable to cal dark current. The SoftMax® Touch Software is installed in. For SpectraMax L or LMax II, LMax II. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Read fluorescence polarization at room temperature (RT, 19–25°C). Please Inquire. 0 out of 5 $ 5,999. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. . Cell viability was assessed by CellTiterGlo (Promega) reagent 96 hours after beginning treatment, and luminescence was measured on a Spectramax M5 spectrophotometer. Choose from a three- (M3), four- (M4), orSpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. With its 5-color monochromator detection head, MOLECULAR DEVICES SpectraMax M5 is the ideal choice for high-throughput applications, providing superior results in the analysis of a. Monday to Friday 7am - 5pm Pacific Time. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Time-Resolved Fluorescence (TRF) Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing. SpectraMax DuoLuc Reporter Assay (SMP 6. Step 4: Incubate at room temperature for 1 hour, protected from light. The drawing shows a standard microplate of 15 mm height covered with a lid. • Tel. Table of Contents. Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. The chamber contains. Microplate Reader Shelf. SpectraMax M5e Multi-Mode Microplate Reader. The chamber contains springs that position the cuvette in the proper alignment for a read. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. View the Molecular Devices websiteSpectraMax® M5 multimode microplate reader from Molecular Devices to measure both reporters in a completely homogeneous manner, in the same well of a 96-well plate, without cell lysis. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 0 mL). For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Multimode Readers Comparison Chart. The assay window spanned four orders of magnitude. Choose cables that meet the requirement described in this appendix. TR-FRET assay principle. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. e. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. Fix: While proper use of the Shake button is to hold the button down for the duration of the desired mixing time, repeated sequential clicking is now allowed. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. SpectraMax® M5/M5e/FlexStation® 3 Instrument Settings Instrument Wavelength Settings EXC Wavelength 575 nm EMS Wavelength 620 nm Auto Cutoff 610 nm Optimized SpectraMax® M5/M5e Settings Readings 10-50 PMT Auto Auto Calibrate On Transcreener® HTS is a universal, high throughput biochemical assayUser guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. 4. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. . 5. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. . Description 1. Description 1. Multimode Reader Comparison Chart. Description. Published Dec 16, 2020 | Updated Dec 16, 2020. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Weight. Molecular Devices also offers a mass spectrometry-based solution for food safety testingMOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Methods Preparation of reagents and standards. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. S. . Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Figure 2. The dual monochromator optics allow. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. $1,250. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. ; The top 3 importers of Spectramax are India with 365. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. The viscosity solution was prepared by mixing PBS and Glycerol in different proportion. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 190-1000. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. 9000-0756. The M5 has two PMTs. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Also refer to this Multimode Reader Comparison Chart. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). NORTH AMERICA. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. For more sample throughput, the system canMolecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. . Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Bookings are free, but you will need to use iLab to book. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Multimode Readers Comparison Chart. Fluorescence intensity was measured using a. FlexStation® 3 Multi-Mode Microplate Reader. 7°C and the bottom to 36. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. For example, microplates containing different samp les can be read using the same orPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Figure 1. In the M2, M5 and Plus 384, the light. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). With optimized reagents, option to operate the. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeWe would like to show you a description here but the site won’t allow us. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. . The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax Quant dsDNA Assay Kits. With optimized reagents, option to operate the readers. Third this recommendation. The SpectraMax M5 is a microplate reader that can be used for various applications,. 72 MB. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The assay applies fluorescence polarization to quantify Fc-containing IgG. 6. 1). Small, unbound molecules rotate rapidly in solution (top), while large,. Features. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:In this application note, we describe how the SpectraMax® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. , ~488 nm), has the optical. clicked with the SpectraMax L Microplate Reader. Bookings are. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. +1 800-635-5577. We would like to show you a description here but the site won’t allow us. . Cet appareil ISM est confomre à la norme NMB-001 du Canada. D 1. SpectraMax M5 device. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. . 0 mm wide, and heights from 14 mm to 15 mm. . 多功能酶标仪 FlexStation3 快速动力学操. The following document is intended to demonstrate setup of this. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode We would like to show you a description here but the site won’t allow us. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 7°C (gradient heating design). Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. HTRF is a proprietary time-resolved fluorescence technology that overcomes SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. . INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. Dual monochromators provide the ability for. 2. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. . HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Softmax Pro GxP Software 6. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. Example: [Drawer]. Fluorescence Microplate Reader Comparison Chart. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Read fluorescence polarization at room temperature (RT, 19–25°C). Sensitive fluorescent quantitation of DNA with the Quant-iT. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Table 1. Microplate Reader Shelf. Multimode Reader Comparison Chart. 3: The cuvette chamber. Wavelength Range. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. The chamber. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 7. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. SpectraMax ABS Plus SpectraTest ABS2 v1. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. For example, microplates containing different samp les can be read using the same orProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Description 1. The following document is intended to demonstrate setup of this instrument. . . The M5 can read volumes down to 2uL with the. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. spr SpectraMax iD5 SpectraTest ABS2 v1. Refer to “Installing Detection Cartridges” in. The following document is intended to demonstrate setup of this instrument. Add to Cart. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 312 gain calibration failed. item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). The following document is. There were 12 replicate wells per dilution in both 96-well and 384- well plates. We would like to show you a description here but the site won’t allow us. For very concentrated samples, use a minimum2. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The integration time suggested is 5-10 sec. Examples: “Press carefully to engage,” “ Do not press down. SpectraMax M5 microplate reader. 4. Other technologies such as imaging. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 2. We would like to show you a description here but the site won’t allow us. 1. In the M2, M5 and Plus. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. When the correct COM port is selected, the. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The following document is intended to demonstrate setup of this. Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. . The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. When the user applies a set temperature of 37°C, the top is heated to 37. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. edu) to be added as a trained user in iLab. Description 1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 4. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. 1step assay with SpectraMax microplate readers. Published Dec 16, 2020 | Updated Dec 16, 2020. Select the fluorescence option and read the total fluorescence. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 1) EarlyTox Glutathione Assay (6. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. The. The plate reader can measure multiple readouts, including UV and visible absorbance. The plate reader can measure multiple readouts, including UV. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 314 low lamp level warning. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 3: The cuvette chamber. 0 out of 5 $ 15,999. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. Optimized for fluorescence microplate readers. Lift up the lid over the chamber to insert or remove a cuvette. Figure 1. Offer Ends In: 18 Days 19: 38: 21. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. Description 1. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Cytokines in medium released. Fluorescence Microplate Reader Comparison Chart. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax L Microplate Reader provides medium to high throughput. SpectraMax M5 and the Analyst GT. These all-inclusive assay kits are ready to use in assay validation or screening. 0200-0729. Customer Satisfaction is our number one goal All lab equipment in stock. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The chamber. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. SpectraMax Glo and DuoLuc. . Operated fromFluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Consistent results are obtained over time offering workflow flexibility. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. . Microplate Readers include the M3, M4,. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. SoftMax Pro. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. Step 4: Incubate at room temperature for 1 hour, protected from light. 1. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Refurbished. Download PDF versions of the SpectraMax M5 User Guides from this page. In either case. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. , ~488 nm), has the optical. 72 MB. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. The. Samples were analyzed in eight to sixteen wells per independent. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Settings not required by a reader are indicated by ‘---’. Add to Cart. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. 5. Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. l. Table of Contents. Purchase Overlord with the required number of. Step 1: Open the SoftMax Pro IMAP FP protocol. Open the Softmax Pro software and select the FP option. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Control Panel The control panel houses the instrument status LEDs and the microplate. used. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. MDS Analytical Technologies announced the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. All reagents were brought to room temperature before use. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Introduction. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Wash Buffer Concentrate was diluted 10-fold. Table of Contents. 7" color, capacitive touch screen. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. . Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. Description. Lift up the lid over the chamber to insert or remove a cuvette. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. 40266 Email: [email protected]® M5多功能读板机为使用者提 供了除化学发光以外的其他检测方法，例 如：光吸收，荧光强度，荧光偏振和时间 分辨荧光。 SpectraMax® L和SpectraMax® M5酶标 仪均用SoftMax® Pro软件进行操控和设 置，该软件含有CellTiter-Glo检测模板和0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. We would like to show you a description here but the site won’t allow us. Choose from a three- (M3), four- (M4), orThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. . Softmax Pro GxP Software 6. 0 out of 5 $ 17,999 $ 16,995. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. 7°C (gradient heating design). Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. SpectraMax M5 Multi-Mode Microplate Reader. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . edu) to be added as a trained user in iLab. D 1. the IMAP Platform and smoothly transition into high-throughput screening. These robust readers have been placed in labs from. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. When a feature or capability applies to only certain readers, this exception is noted. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Description 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. no. 6. 2. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument.